The Mane Six is a group of Ponies, and barers of the Elements of Harmony placed by Celestia to protect Equestria and to spread the magic of friendshuip to others. The group was involved in numerous incidents in Equestria, and saved the world from a number of threats, including Nightmare Moon, Sombra, and Tirek, along with threats on Earth and Homeworld since their first meeting with the Crystal Gems.
- Twilight Sparkle - She is considered the leader of the Mane Six, and the only Alicorn of the group. She was the student under Princess Celestia herself, and represents the Element of Magic.
- Applejack - Apart from the extensive Apple Family, Applejack is an Earth Pony the muscle of the group and the main voice of reason to keep the group together alongside Twilight. She represents the Element of Honesty.
- Fluttershy - She's a nimble Pegasus that moved to Ponyville. As to her Cutie Mark, she is able to communicate with other animals, and often takes care of them. She represents the Element of Kindness.
- Pinkie Pie - Out of all the members, Pinkie Pie is the most bombastic and childish, often presented as comic relief and extreme dynamics. She represents the Element of Laughter.
- Rainbow Dash - The fastest flyer from Cloudsdale, she is the most action-oriented member of the group. She's most notable for the Sonic Rainboom, and what had brought her friends all their cutie marks, hers included. She represents the Element of Loyalty.
- Rarity - She is a Unicorn, the fashionista of the group, and runs her own fashion boutique within Ponyville. She represents the Element of Generosity.
- Starlight Glimmer - A high-expert Unicorn who once believed equality was the key to Harmony, and the former enemy to the team, Twilight helped her see the flaw in her ways and had her join her team to regain Friendship.
- Crystal Gems - They first met the Crystal Gems during the "Everfree Forest invasion". They're most often aiding them in numerous missions both on Earth and on Homeworld.